instrumentation

Laboratory automation system overview

Thermo Fisher Momentum software

facilitates scheduling of multiple experiments simultaneously

Thermo BenchTrak Orbitor

automated 360-degree plate manipulator with internal regrip station, mounted on a 3.6 m linear track; used to integrate various other pieces of wet lab equipment into a single automation system

BioNex HiG4 robotic centrifuge

microplate centrifugation, up to 5000g

4x Inheco Incubator-Shaker DWP

bacterial culture and incubation in 96-well deepwell plates

Agilent PlateLoc

automated plate sealer

Agilent VCode

automated barcode printing and application for microplates

Thermo Cytomat Hotel

for random access to hundreds of microplates

Thermo MultiDrop Combi reagent dispenser

for rapid filling of microplates with common reagents

Opentrons OT-2

small, customizable liquid handling robot controlled using open-source Python code

Tecan Freedom EVO 200

automated liquid handling workstation equipped with air and liquid liquid-handling arms, robotic manipulator arm, vacuum manifold, heating/shaking stations, and Pelteir modules

Tecan HP D300e

direct digital titration of compounds in DMSO

Tecan Infinite M1000PRO multifunction plate reader

equipped for absorption, fluorescence, fluorescence polarization, luminescence, and alphascreen assays, with injectors for kinetic assays

Tecan SPARK

equipped for absorption, fluorescence, fluorescence polarization, and luminescence, with injectors for kinetic assays and cooling module

Roche LightCycler 480

qPCR for standard PCR and thermofluor protein denaturation melts

ServersCheck

temperature/humidity probes

Tecan Freedom EVO 200 liquid-handling workstation

Liquid Handling Arm (LiHa)

liquid-based syringe-actuated pipetting arm for dispensing volumes up to 1000 uL without the need for disposable pipette tips

Air LiHa

air based pipetting arm with disposable tips for accurate dispensing of 1-200 uL volumes

Tecan Te-VacS

vacuum station with two independently controlled vacuum manifolds

4x Torrey Pines EchoTherm RIC20 Peltier units

for heating and cooling

Robotic Manipulator Arm (RoMa)

robotic manipulator arm for manipulating labware on the deck

2x Inheco Thermoshake heating cooling shakers

for heating, cooling, and shaking while pipetting

Compound stock solution preparation/Compound storage

LabMinds Revo automated buffer maker (now called accroma bufferPrep)

fully automated, reproducible, traceable preparation of buffers in liter quantities, requested by built-in tablet or on-the-go from your mobile device

Roylan Developments Ltd StoragePods

multichannel compound storage unit to prevent moisture absorption of samples; storage capacity of 252 shallow well plates or 72 tube racks

Mettler-Toledo Quantos automatic gravimetric solution preparation system

powder and liquid dosing heads enable accurate preparation of compound solution stocks; integrated Mettler-Toledo QD206DR balance has 0.005 mg @ 81 g / 0.01 mg @ 220 g readability

Column chromatography

Four-tiered Bio-Rad NGC Quest 10 Plus Chromatography System

for column chromatography; equipped with 10 mL/min pumps, multi-wavelength (UV/Vis) and conductivity detectors, pH sensing, automated sample injection, buffer blending valve, with the capacity to run multiple columns in succession

Fisherbrand Dispensing Peristaltic Pump

to filter solutions before applying to chromatography systems

Thermodynamic measurement

Malvern MicroCal Peaq-ITC Automated

for automated thermodynamic measurements of biomolecular interactions with the capacity to run up to four 96-well plates per experiment

Density measurement

Mettler-Toledo DM40 density meter

with 0.0001 g/cm3 accuracy, 0-3 g/cm3 range, and temperature measurement range of 0-91 C using 1 mL sample volumes

Mettler-Toledo

SC30 automated sample changer with 30-sample capacity

LabX

ELN software for data management